3D Deconvolution
IMAGES

DAPI Images Meiotic Prophase Chromosomes
optical sections through maize pollen mother cells

• STAGES, Annotated substages of meiotic prophase
• A-F white on black, Closeup of optical section through 6 nuclei.
• A-F black on white, inverted of above

• Maize Bouquet
  (Murphy, Bass), maize line A344+ at the bouquet stage, as seen by telomere FISH, from 3-D, three-wavelength image.
• Maize Bouquet
  (Bass, Sedat, Cande), as seen by telomere FISH, from 3-D, two-wavelength image.
• Telomere and 5S rDNA FISH at pachytene with DAPI plus two FISH probes
  (Bass, Sedat, Cande) Same nucleus, different sections and pseudo-coloring
  6sa2.JPG, Blue=DAPI, Green=Telomere-FITC, and Red=5srDNA-Rhodamine.
  6sa3.GIF, Red=DAPI, Green=Telomere-FITC, and Blue=5srDNA-Rhodamine
• Maize Inbred Line A344 has a conspicuous bouquet
  (Bordoli, Bass, FSU)
  Most maize lines do not reveal the bouquet structure in DAPI-stained chromosome images. A344 + is an exception. Here are various views (projections) of a single nucleus at late zygotene.
  DAPI Image - DNA, the bouquet is at 11 o'clock and knobs are evident
  SPIN ME A344-bq-DAPI.mov 360 movie

  FITC Image - Telomeres; telomere cluster is evident
  SPIN ME A344-bq-FITC.mov 360 movie

  RHOD Image - knobs; direct labeled rhodamine oligo probes for knobs, NUBI-R, stains knob sequence clusters, including small telomeric clusters.
  SPIN ME A344-bq-RHOD.mov 360 movie

  3 Color Overlay; shows DAPI (red), FITC-telomeres (green), and Rhodamine (white-knobs). Scale bars are 5 micron.
  SPIN ME A344-bq-color.mov 360 movie
  

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