3-D reconstruction of stage-4 egg chamber with Hnt misexpression using Delta-Vision photomicroscopy. Hnt-misexpressing follicle cells (marked by GFP, shown in green) had much larger nuclei than the adjacent wild-type follicle cells, indicating a premature entry into the endocycle. The nuclei were marked by DAPI (shown in blue), as well as nucleolus membrane protein lamin (shown in red).