Patch-clamp Electrophysiology and Site-directed Mutagenesis


Lecture Outline:
I.  General Background on Bioelectric Signals
II.  Acquisition of Data
III.  Biological Preparations
IV.  Parallel Tools
	A.  Heterologous Expression Systems
	B.  Site-directed Mutagenesis
	C.  Protein Biochemistry

Literature Resources:
You may check any of these out from my office library....
Literature is listed in order of increasing difficulty.

Levitan and Kaczmarek.  1997.  The Neuron.  Oxford Press.
New York, New York.

Axon Instruments.  1993.  The Axon Guide for Electrophysiology
and Biophysics Laboratory Techniques.  Foster City, CA.

Gerrish, Dugger, and Roberts.  1999.  Electricity and Electronics.
Goodheart-Wilcox Company, Inc. USA.

Hille, B.  2001.  Ionic Channels of Excitable Membranes.  Sinauer
Associates.  Sunderland, MA.

Sakmann and Neher.  1995.  Single-channel Recording.  Second Edition.
Plenum Press.  New York, New York.

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