Maroun J. Beyrouthy
Ph.D. Molecular and Cell Biology, 2006, Florida State University
PhD Advisor: Dr. Myra M. Hurt
Biology Department, Florida State University
Department of Biomedical Sciences
Tallahassee, FL-32306
850-645-2931
Research Interests
Currently, I am considering post-doctoral opportunities in two areas of interest to me:
* Cancer research in human cells, or
** Stem cell research: "cancer-stem cell hypothesis".
Education
1994, French Baccalaureat. Mont-La-Salle, Beirut, Lebanon
1994-1998, B.S. Biology. American University of Beirut (AUB), Beirut, Lebanon
1998-1999, Research Assistant. Biology Dept., AUB
1999-2001, Kursk State Medical University. Kursk, Russia
2001-present, Ph.D. candidate. Florida State University, Tallahassee, Florida
2006, Ph.D. in Biological Science. Florida State University, Tallahassee, Florida
PhD Project
Research in our lab centers on gene regulation in the mammalian cell cycle. We are specifically interested in studying G1 and G1-S events. I have been involved in different ongoing projects to understand the upregulation of histone genes at the onset of S phase. One includes the subcellular localization of YY1 as being among the factors required for the expression of the replication-dependent histone genes. Moreover, I am doing protein purification of a protein complex (omega) shown also to regulate the same family of histone genes. I use various research techniques from mammalian cell culture to EMSA, Western blot, immunocytochemistry (slide show, slide show2 ), immunoprecipitation, RTP and confocal microscopy.
And most recently, I am involved in a microarray experiment and its subsequent analysis which aims at profiling, on a genome-wide scale, the gene activity in G1 and S phase of the human cell cycle.
In September of 2002, I spent two weeks at the Stanford Biomedical Research Center, CA. During this training period, I learned to amplify RNA samples, Cy3/Cy5 direct labeling of those samples by reverse transcriptase, and their hybridization to glass cDNA microarrays containing more than 43,000 genes elements, in addition to scanning and gridding of the corresponding 43K arrays slides.
Also, I attended the SMD tutorial. The Stanford Microarray Database is a powerful online tool to store, retrieve, filter, and cluster microarray data.
** In the news!
Yin Yang 1 (YY1) & Histone Gene Expression
Meeting Presentations and Seminars
Poster. “YY1, Transcription, Cell Proliferation & Cell Death”. Mechanisms of Eukaryotic Transcription. Cold Spring Harbor Laboratory, NY.
Talk. “Specific Effects on the Transcription Factor YY1 in Apoptotic Cells”. Experimental Biology. San Diego Convention Center, San Diego, CA.
Research Talk. Seminar series organized by Dr. Marwan El-Sabban. Medical School. AUB, Beirut, Lebanon.
Talk. Microarray Seminar. Series organized by Dr. Dan McGee. Statistics Dept., FSU.
Poster. "The Yin Yang-1 (YY1) protein undergoes a DNA replication-associated switch in localization from the cytoplasm to the nucleus at the onset of S phase". Miami Winter Symposium. "The Cell Cycle, Chromosomes and Cancer". Miami Beach, FL. [Report]
Structural Biology Symposium. FSU, Tallahassee.
Talk. Microarray Seminar. Biology Dept., AUB. Beirut, Lebanon.
Poster."Coordinate Regulation of YY1 Subcellular Localization and DNA Synthesis". Mechanisms of Eukaryotic Transcription. Cold Spring Harbor Laboratory, NY. [Abstract] [Poster]
Talk. "Effect of Organochlorine mixtures on reproduction in rats". The 14th Science meeting, Lebanese association for the advancement of science. Department of Engineering (AUB). Beirut, Lebanon.
CSHL Conference Fellowship. Cold Spring Harbor Conference, Cold Spring Harbor, NY. September, 2005
Paper Award and Travel Award. American Society for Biochemists and Molecular Biologists, San Diego, CA. April, 2005
Bennison Memorial Award, Department of Biological Sciences, Florida State University, 2002
Publications
Beyrouthy M.J., Alexander K.E., Baldwin A., Whitfield M.L., Bass H.W., and Hurt M.M. Mitotic selection of normally cycling cells in culture. (Submitted for publication)
Krippner-Heidenreich A., Walsemann G., Beyrouthy M.J., Speckengs S., Kraft R., Thole H., Talanian R.V., Hurt M.M. and Luscher B. Caspase-dependent regulation and subcellular redistribution of the transcriptional modulator YY1 during apoptosis. Mol Cell Biol. 2005; 25(9):3704-14.
Palko L., Bass H.W., Beyrouthy M.J., and Hurt M.M. The Yin Yang-1 (YY1) protein undergoes a DNA replication-associated switch in localization from the cytoplasm to the nucleus at the onset of S phase. Journal of Cell Sci. 2004; 117(3):465-476. [pdf upon request]
Tarraf C., El-Sabban M., Bassam R., Beyrouthy M. , Chamoun J., Talhouk R. Functional consequence of exposure to dieldrin on mammary development and function. Food Addit Contam. 2003; 20(9):819-828. [pdf upon request]
Created by Maroun Beyrouthy
In place Dec, 2002.
Updated December, 2006
Pictures on this site courtesy of BMJ. Please do not duplicate without owner permission!
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