November 2006

 

 

KENNETH H. ROUX

 

           I.PERSONAL

 

            Address:

                Department of Biological Science

                Biology Unit I, Rm. 335

                Florida State University

                Tallahassee FL 32306-4370

                Phone  (850) 644-5037

                Fax:     (850) 644-0481

                E-mail: roux@bio.fsu.edu

 

          II.EDUCATION AND DEGREES

 

               1966–1970   Delaware Valley College, Doylestown, Pennsylvania

                        Degree received:  B.S., Biology

 

               1970–1972   Tulane University, New Orleans, Louisiana

                        Degree received:  M.S., Biology

 

               1972–1974   Tulane University, New Orleans, Louisiana

                        Degree received:  Ph.D., Biology

                        Dissertation director:  E. Peter Volpe

 

         III.FELLOWSHIPS AND TRAINEESHIPS

 

            National Science Foundation Graduate Traineeship, 1970-1974

            National Research Service Award — NIH Postdoctoral Fellowship, 1975–1977

 

        IV.ACADEMIC AND RESEARCH POSITIONS

 

               1974–1975Instructor, Tulane University, New Orleans, Louisiana

 

               1975–1978     NIH Postdoctoral Fellow, Instructor, University of Illinois,                                    Medical Center, Chicago, Illinois, Department of                                     Microbiology

 

               1978–1983Assistant Professor, Florida State University, Department of                                     Biological Science, Tallahassee, Florida

 

               1983–1988Associate Professor, Florida State University, Department of                                     Biological Science, Tallahassee, Florida

 

            1988–present  Professor, Florida State University, Department of Biological                                     Science and Institute of Molecular Biophysics,                                            Tallahassee, Florida

 

 

V.      RESEARCH INTERESTS

 

Structural Analysis of the AIDS Viruses, HIV-1 and SIV, and the Antibodies That Neutralize Them

 

We have used negative stain electron microscopy and cryoelectron microscopy coupled with tomography to produce the first detailed 3-D images of the surface of the AIDS viruses.  The information gained is of fundamental importance to the development of AIDS vaccines and other therapeutic agents and our understanding of the function of the viral surface envelope spikes.  We are currently focusing on the mechanism by which certain antibodies are able to neutralize HIV-1 and are studying the surface of the virus itself to determine how these antibodies bind.  During this process, we contributed to the discovery of an unprecedented form of antibody in which a bizarre structural anomaly has resulted in the formation of a potent neutralizing antibody that binds to the carbohydrates that are used by the virus to escape detection. 

 

Food-Allergen Characterization and Immunoassay Development

 

An overactive immune system can sometimes generate antibodies with the potential to do harm, as is the case in allergic reactions.  In this arena, we (in collaboration with Dr. Shridhar K. Sathe, Nutrition, Food and Exercise Sciences, FSU, and Dr. Suzanne Teuber, UC Davis Medical School) are investigating the mechanisms by which antibodies arise to certain food molecules, specifically tree-nut allergens.  Such antibodies can lead to sometimes-fatal reactions upon allergen ingestion.  We have characterized several allergens from cashew, almond, and walnut to date and are pursuing additional allergens from these and other tree nuts.  In addition we have developed antibody-based tests for tree nuts, which can be used by the food industry and regulatory agencies to monitor for product contamination.

 

 

        VI. PROFESSIONAL HONORS

 

                    Awarded, Loretta C. Ellias Award in Recognition of Excellence in Undergraduate Teaching, Department of Biological Science, FSU, 1996

 

                    Awarded, Alumni Association Achievement Award in Science, Delaware Valley College, 2003

 

            Named, Distinguished Research Professor, Florida State University, 2005

 

            Named, Kurt G. Hofer Professor of Biological Science, 2005

 

 

 

 

       VII.        GRADUATE STUDENTS, POSTDOCTORAL ASSOCIATES, VISITING SCIENTISTS

 

               Past Graduates:

 

                        Scott M. Laster, M.S. 1981, Ph.D. 1984, Genes of the rabbit VH subgroup III. Currently: Professor, Department of Microbiology, North Carolina State University, Research interests: Molecular biology of T cells and macrophages.

                        Wayne T. McCormack, M.S. 1983, Ph.D. 1987, Rabbit immunoglobulin VH genes: molecular basis for latent allotype expression. Currently: Associate Dean for Graduate Studies, Interdisciplinary Program, School of Medicine, and Professor, Department of Pathology and Lab Medicine, School of Medicine, University of Florida. Research interests: Molecular genetics of human and avian T cell receptors.

                        Mohsen Abolhassani, Ph.D. 1984, Biochemical and genetic characterization of the rabbit heavy chain VHa and VHy subgroups. Currently: Assistant Professor, Department of Immunology, Pasteur Institute of Iran, Teheran, Iran, Founding Editor, Iranian Biomedical Journal, Medicine, Lymphokine modulation in cancer. Research interests: Immunobiology of Leishmania infections. 

                        Brian McClain, M.S. 1987, Network regulation of antibody production via rabbit allotype and idiotype interactions. Currently: Leon County Florida high school science teacher.

                        Randall W. Renshaw, M.S. 1989, Evidence for a1 germline VH genes encoding latent VHa1 allotypes in a VHa2 rabbit. Molecular biology of Ig diversity,.

                        Catherine M. Rossi, M.S. 1990, The development of an expression system for the detection of immunoglobulin gene products. Currently: Physician, Dell Ray Beach, Florida,

                        Carol Leggett, M.S. 1991, Investigation of latent allotype expression in the rabbit. Currently; Instructor, Valdosta State University.

                Bo Xu, M.S. 1992, Comparison of gene organization in a1a1 and a2a2 rabbits. Ph.D., M.D, Tufts University, Massachusetts, Currently: Assistant Prof. U. Massachusetts Medical School, Dept. Pathology. Sequence analysis of rabbit VH genes.

                Lei Hu, M.S. 1996, Characterization of the recombination-activating gene, RAG-2, mRNA of the rabbit. Currently: Staff Scientist, Department of Biological Science, FSU.

                    Martin Acosta, M.S. 1998 (co-director, SK Sathe, thesis director), Characterization of rabbit IgG polyclonalantibodies raised against the major protein in almond (Prunus amygdalus L.), Currently EPA staff scientist.

                        Yanhong Wei, M.S. 2002, Development of an immunoassay for the detection of cashew in foods. Currently: Research Associate, Antibody Department, Human Genome Sciences, Inc., Rockville, MD

                        Mahesh Venkatachalam, PhD 2004 (co-director, SK Sathe, dissertation director). Chemical composition of select pecan [Carya illinoinensis (Wangenh) K. Koch] varieties and antigenic stability of pecan proteins. Currently: Post-Doctoral Research Associate, Purdue Univ., W. Lafayette, IN

                        Pallavi Tawde. M.S. 2004, Investigations of the cross-reactivity between cashew and pistachio in allergic patients. Currently, PhD candidate.

                        Jason Robotham, PhD, 2006, Identification, characterization and epitope mapping of tree nut allergens. Currently, postdoctoral associate with Hengli Tang, Biology, FSU, Investigations of hepatitis C viruses.

 

                        Current Graduate Students:

 

                        Pallavi Tawde, August 2000–present, PhD candidate

                        Henry Grise, Sept, 2002-present, PhD candidate

                        LeAnna Willison, August 2006-present, M.S. candidate

 

 

                        Former Postdoctoral Associates:

 

                Karl Hecker, 1993–1995, Investigating the basis for touchdown PCR. From: Florida State University, Department of Chemistry.  Currently:  Director, Applied Research, Invitrogen, Inc. Carlsbad, CA.

                        Lioudmila Strelets, 1994–1997. Structural analysis of immunoglobulin flexibility. From: Biophysics Program, Electron Microscopy Laboratory, Koltzoud, Russia, Institute of Molecular Biology.  Currently:  Research Associate, University of North Carolina, Chapel Hill, NC.

                        Fang Wang, August 2000–September 2002.  Cloning of tree nut allergens.  Currently: Research Associate, P. B. Chase lab, Department of Biological Science, FSU.

 

                        Current Postdoctoral Associates:

 

                Ping Zhu, March 1999–present. HIV and SIV structural studies.

                        Lauren Porter, August 2004-present. HIV and SIV structural studies.

 

 

                        Former Visiting Scientists:

 

                        Roald Nezlin, Weizmann Institute Rahovot, Israel, May 1993.

                        Shridhar K. Sathe, Department of Nutrition, Food & Exercise Science, FSU (sabbatical visit), Spring 2002.

                        Yeldur Venkatesh, Principal Scientific Officer, Department of Biochemistry and Nutrition, Central Food Technology Research Institute, Mysor, India (one year sabbatical visit), 2003-2004.

                         Mohsen Abolhassani, Senior Scientist, Pasteur Institute of Teheran, Iran (one year sabbatical visit). 2004-2005.

 

 

      VIII.HONORS AND UNDERGRADUATE RESEARCH STUDENTS

 

            High School Summer/Intern Programs:

                Andrew Segal, 1983

                Andrew Fitch, 1983

                Craig Morris, 1996

 

            Honors:

                    Joel Bumgardner, 1989

                Jeffrey Melendez, 2000

 

            Directed Individual Studies:

                Greg Niely, 1984

                Don Benson, 1985

                  Carol Leggett, 1986–87

                Kimbal Frazer, 1991–92

                Michael Bergman, 1993

                Patrick Johnston, 1993

                Paul Philpott, 1994

                Leigh St. Petery, 1994-95

                  Jabal Uffelman, 1995

                Martin Acosta, 1995

                  Chris Delcher, 1995

                Jeff Ward, 1996–97

                Jennifer Womer, 1998

                Bryan Galo, 1998

                Ferrel Long, 1998

                Genevieve Dulan (FAMU), 1998-99

                Pallavi Gadwal, 2002

                Dillon Fritz, 2002–2003

                 Jordan Smith 2006-

                 Leah Williams 2006-

 

            Summer American Cancer Society Fellowships:

                Damon MacMillan, 1992

                Bryan Galo, 1998

 

            Undergraduate Research Assistants:

                Henrike Froemke, 1992

                Jason Howard, 1993–94

                Richard Penney 2002

 

            Honors Student:

                Jeffery Melendez, 1999–2000

 

 

IX. PUBLICATIONS

 

Book Chapters:

 

1.  Greenspan, N.S. and Roux,. K.H., l988.  Categories of idiotope overlap and anti-idiotypic memory of antigen.  In: Theoretical Immunology, Part Two, Studies in the Science of Complexity.  Addison-Wesley, Reading. p. 215 - 231.

 

2.  Roux, K.H. and Tankersley, D., 1990.  Analysis of spontaneous Id anti-Id dimers, in pooled human IgG.  In:  Idiotype Networks in Biology and Medicine.  Excerpta Medica, Amsterdam, 1990. P. 43 – 49.

 

3.                  Roux, K.H.  Optimization and troubleshooting in PCR.  In:  PCR Primer:  In:  A Laboratory   Manual. C. W. Dieffenbach and G. S. Dveksler, eds., Cold Spring Harbor Press, pp53-62. 1995.

 

4.                  Roux, K.H. and Mage, R.G.  Rabbit immunoglobulin allotypes.  In:  Handbook of Experimental Immunology.  Weir et al. eds., Blackwell, pp. 26.1-26.17, 1996.

 

5.                  Roux, K.H. and Hecker, K.H.  One step optimization using Touchdown and Stepdown PCR. In:  PCR Cloning Protocols.  White, B.A., ed., Humana Press, pp. 39-45, 1996.

 

6.                  Roux, K. H. And Greenspan, N. S.  An electron microscopic view of the geometry and dynamics of idiotype-anti-idiotype interactions. In:  Idiotypes in Medicine - Infections, Autoimmunity and Cancer. Shoenfeld, Kennedy and Ferrone, eds., Elsevier Science, Amsterdam, pp. 281-291, 1997.

 

7.                  Roux, K. H.  Direct examination of immunoglobulin and small immune complexes by negative-stain immunoelectron microscopy.  In:  Immunology Methods Manual, Lefkovits, ed., Academic Press, pp. 8.6.1-8.6.10. 1997.

 

8.                  Roux, K.H. and Hecker, K.H.  One-step optimization using touchdown and stepdown polymerase chain reaction. In:  The Nucleic Acid Protocols Handbook, R. Rapley, ed., Humana Press, Totowa, NJ, pp. 589-594. 2000.

 

9.                  Roux, K.H.  Single-step optimization using Touchdown and Stepdown PCR Programming,       In:  PCR Cloning Protocols, 2nd ed..  B-Y. Chen and H. W.  Janes., eds., Humana Press, pp. 31-36. 2002. (co-listed and co-published as journal paper)

 

10.             Roux, K. H.  2003.  Optimization and troubleshooting in PCR.  In:  PCR Primer:  A Laboratory Manual. 2nd ed. C. W. Dieffenbach and G. S. Dveksler, eds.  Cold Spring Harbor Press, Plainview, NY.  pp. 35–41.  2003.

 

 

Research Papers:

 

11.             Roux, K.H., and Volpe, E.P.  Expression of histocompatibility loci in the leopard frog.  J. Heredity 65: 341-344, 1974.

 

12.             Roux, K.H., and Volpe, E.P.  Evidence for a major histocompatibility complex in the leopard frog.  Immunogenetics 2: 577-589. 1975.

 

13.             Horng, W.J., Gilman-Sachs, A., Roux, K.H., Molinaro, G., and Dray, S. Auto-antibody to an Ig VH region allotype:  Induction of anti-al antibody in an al-suppressed ala2 heterozygous rabbit.  J. Immunol. 119: 1560-1562, 1977.

 

14.             Roux, K.H., Metzger, D.W., and Dray, S.  Suppression and enhancement of the rabbit primary in vitro antibody response with anti-allotype antibody:  Concurrent effects on thymidine incorporation,  blastogenesis and cell surface Ig.  J. Immunol. 122: 566-574, 1979.

 

15.             Roux, K.H., Gilman-Sachs, A., and Dray, S.  Idiotypic specificities which arise spontaneously in rabbits following allotype suppression of the major VH subgroup.  Eur. J. Immunol. l0: 526-529, 1980.

 

16.             Roux, K.H., Gilman-Sachs, A., and Dray, S.  The identification of a VH subgroup allotypic specificity, y30, which differentiates the y33 allele into two variants:  Implications for rabbit VH gene  organization and evolution.  Molec. Immunol. 18: 359-365, 1981.

 

17.             Roux, K.H.  A fourth heavy chain variable region subgroup, w, with two variants defined by an induced auto-antiserum in the rabbit.  J. Immunol. 127: 626-632, 1981.

 

18.             Horng, W.J., Roux, K.H., Gilman-Sachs, A., and Dray, S.  An increase in the number of recombinant molecules and other effects of the simultaneous allotype suppression of trans-chromosomal a VH and n C Ig gene products.  Molec. Immunol. 19: 15l-158, 1982.

 

19.             Gilman-Sachs, A., Roux, K.H., Horng, W.J., and Dray, S.  Characterization of additional rabbit IgM allotypes and the effect of suppression of a VH locus allotypes on the expression of n C. locus allotypes.  J. Immunol. 128:451l-456, 1982.

 

20.             Metzger, D.W., and Roux, K.H.  A predominant idiotype on rabbit anti-VH al allotype antibodies is shared by both major and minor VH subgroups.  J. Immunol. 129: 1138-1142, 1982.

 

21.             Roux, K.H., Drake, J., Edmond, J., and Sheperd, R.  Domestic rabbits and wild Australian rabbits display similar variable heavy-chain haplotypes.  Immunol. Letters 5: 117-120, 1982.

 

22.             Roux, K.H., and Metzger, D.  Immunoelectron microscopic localization of idiotypes and allotypes on immunoglobulin molecules.  J. Immunol. 129:  2548-2553, 1982.

 

23.             McCormack, W., and Roux, K.H.  Monoclonal antibody specific for b5 allotype of rabbit kappa light chains.  Hybridoma 2: 97-107, 1983.

 

24.             Laster, S.M., and Roux, K.H.  Response of the rabbit VH subgroups a and y following antigenic stimulation.  Immunology 49: 273-279, 1983.

 

25.             Roux, K.H., Kilpatric, J.M., Volanakis, J.E., and Kearney, J.F.  Localization of the phosphocholine-binding sites on C-reactive protein by immunoelectron microscopy.  J. Immunol. 131: 2411-2415, 1983.

 

26.             Roux, K.H.  Regulation of rabbit immunoglobulins.  Introductory Remarks.  Surv. Immunol. Res. 2: 341, 1983.

 

27.             Roux, K.H.  Rabbit latent allotypes:  current status and future directions.  Surv. Immunol. Res. 2: 342-350, 1983.

 

28.             Roux, K.H., and Metzger, D.W.  Crossreactive idiotypes on heterologous anti-allotype antibody.  J. Immunol. 132: 1341-1345, 1984.

 

29.             Roux, K.H.  Direct demonstration of multiple VH allotypes on rabbit Ig molecules:  allotope characteristics and Fab arm rotational flexibility revealed by immunoelectron microscopy.  Europ. J. Immunol.  14: 459-464, 1984.

 

30.             Roux, K.H., Metzger, D.W., Kazdin, D.S., and Horng, W.J.  Induced latent allotypes within rabbit anti-crossreactive idiotype reagents.  Direct immunoelectron- microscopic evidence.  Europ. J. Immunol. 14: 910-915, 1984.

 

31.             Roux, K.H., McCormack, W.T., and Dhanarajan, P.  A reevaluation of rabbit anti-allotype antibody for the presence of cross-reactive idiotypes.  I. A species-specific idiotype on rabbit anti-a1l antibody is recognized by guinea pig anti-IdX antibody.  J. Immunol. 135: 1961-1966, 1985.

 

32.             Roux, K.H., McCormack, W.T., and Dhanarajan, P.  A reevaluation of rabbit anti-allotype antibody for the presence of cross-reactive idiotypes.  II.  Expression of rabbit a1-like images on goat antibody after immunization with anti-al antibody.  J. Immunol. 135: l967-l974, l985.

 

33.             Abolhassani, M., Fiore, G., and Roux, K.H.  Comparative tryptic peptide analysis of rabbit heavy chain allotypes by HPLC.  J. Liquid Chromatog., 8:2649-2662, 1985.

 

34.             McCormack, W.T., Laster, S.M. Marzluff, W.F., and Roux, K.H.  Dynamic gene interactions in the evolution of rabbit VH genes.  Nucl. Acids. Res., 13:7041l-7054, 1985.

 

35.             Abolhassani, M. and Roux, K.H.  Serologic and biochemical analysis of latent a1 IgG. Molec. Immunol., 23:533-540, 1986.

 

36.             Ueda, A., Kearney, J.F., Roux, K.H., and Volanakis, J.E.  Probing functional sites on complement protein B with monoclonal antibodies.  Evidence for C3b-binding sites on Ba.  J. Immunol. 138:1143-1149, 1987.

 

37.             Roux, K.H., Monafo, W.J., Davie, J.M. and Greenspan, N.S.  Construction of an extended three-dimensional idiotope map by electron microscopic analysis of idiotope-anti-idiotope complexes.  Proc. Natl. Acad. Sci., 84: 4984-4988, 1987.

 

38.             McClain, B.R and Roux, K.H.  Mouse monoclonal antibody bearing a VH framework region determinant similar to a rabbit VHa1 allotype.  Immunology, 61: 397-402, 1987.

 

39.             Rudich, S.M., Roux, K.H., Winchester, R.J., and Mongini, P.K.A.  Anti-IgM-mediated B cell signaling:  Molecular analysis of ligand binding requisites for human B cell activation and tolerance.  J. Exp. Med. 168: 247-266, 1988.

 

40.             Davis, A.C., Roux, K.H., and Shulman, M.J.  On the structure of polymeric IgM.  Europ. J. Immunol. 18: 1001-1008, 1988.

 

41.             McCormack, W.T., Dhanarajan, P., and Roux, K.H.  Comparison of latent and nominal rabbit Ig VHa1 allotype cDNA sequences.  J. Immunol. 141: 2063-2071, 1988.

 

42.             Roux, K.H.  Immunoelectron microscopy of idiotype-anti-idiotype complexes. Methods in Enzymology. 178: 130-144, 1989.

 

43.             Davis, A.C., Roux, K.H., Pursey, J. and Schulman, M.J.  Intermolecular disulfide bonding in IgM:  effects of replacing cysteine residues in the µ heavy chain.  EMBO J. 8: 2519-2526, 1989.

 

44.             Roux, K.H. and Dhanarajan, P.  A strategy for single site PCR amplification of dsDNA:  priming digested cloned or genomic DNA from an anchor-modified restriction site and a short internal sequence.  BioTechniques 8: 48-57, 1990.

 

45.             Roux, K.H. and Tankersley, D.L.  A view of the human idiotypic repertoire:  electron microscopic and immunologic analyses of spontaneous idiotype-anti-idiotype dimers in pooled human IgG.  J. Immunol. 144: 1387-1395, 1990.

 

46.             Weisman, H.F., Bartow, T., Leppo, M.K., Marsh, H.C., Carson, G.R., Concino, M.F., Boyle, M.P., Roux, K.H., Weisfeldt, M.L. and Fearon, D.T. Soluble human complement receptor type 1:  In vivo inhibitor of complement suppressing post-ischemic myocardial inflammation and necrosis.  Science 249: 146-1511, 1990.

 

47.             Roux, K.H., Dhanarajan, P., Gottschalk, V., McCormack, W.T. and Renshaw, R.W. Latent a1 VH germline genes in an a2a2 rabbit:  evidence for gene conversion at both the germline and somatic levels.  J. Immunol. 146: 2027-2036, 1991.

 

48.             Fuschiotti, P., Harindranath, N., Mage, R.G., McCormack, W.T., Dhanarajan, P., and Roux, K.  Recombination activating genes-1 and -2 of the rabbit:  cloning and characterization of germline and expressed genes.  Mol. Immunol.  30: 1021-1032, 1993.

 

49.             Roux, K.H.  Using mismatched primer-template pairs in touchdown PCR.  BioTechniques.  16: 812-814, 1994.

 

50.             Roux, K.H. and Greenspan, N. S.  Monitoring the formation of soluble immune complexes composed of idiotype and anti-idiotype antibodies by electron microscopy. Mol. Immunol.  31: 599-606, 1994.

 

51.             Elliott, J.K., Mariscal, R.N., and Roux, K.H.  Do anemone fishes use molecular mimicry to avoid being stung by host anemones?  J. Exp. Mar. Biol. Ecol. 179: 99-113, 1994.

 

52.             Roux, K.H., Shuford, W.W., Finley, J.W., Esselstyn, J., Pankey, S., Raff, H.V., and Harris, L.J.  1994.  Characterization of biosynthetic IgG oligomers resulting from light chain variable domain duplication.  Mol. Immunol. 31:933-942, 1994.

 

53.             Chiu, K.Y., Harauz, G., Greenspan, N.W., and Roux, K.H.  Structured object representation of biomolecular images for two-dimensional shape discrimination via feedforward networks.  J. Computer-Assisted Microscop. 6: 139-147, 1994.

 

54.             Roux, K.H.  Optimization and troubleshooting in PCR.  PCR Meth. and Appl.  4: S185-S194, 1995.  

 

55.             Hecker, K.H. and Roux, K.H.  High and low annealing temperatures increase bothspecificity and yield in touchdown and stepdown PCR.  BioTechniques  20: 478-485, 1996.

 

56.             Roux, K.H.  Negative stain immunoelectron microscopic analysis of small macromolecules of immunologic significance.  Methods  10: 247-256, 1996.

 

57.             Roux, K.H.  Immunoelectron microscopy.  Methods 10: 175, 1996.

 

58.             Williams, R. C. Jr., Malone, C.C., Cimbalanik K., Presley, M.A., and Roux, K.H. Strelets, L. and Silvestris, F.  Human IgG anti-F(ab’)2 antibody cross reacts with DNA and other nuclear antigens.  Arthritus and Rheumatism 40: 109-123, 1997.

 

59.             Hu, L., Epstein, L.M., and Roux, K.H.  Rabbit RAG-2 UTRs: characterization of an unusually large 3’ untranslated region. Immunogenetics 46: 267-275, 1997.

 

60.             Roux, K.H. Strelets, L., and Michaelsen, T.E.  Molecular Flexibility of Human IgG Subclasses  J. Immunol. 159: 3372-3382, 1997.

 

61.             Ellington, W. R., Roux, K. H. and Pineda, A.  Origin of octameric creatine kinases. FEBS Letters 425: 75-78, 1998.

 

62.             Roux, K. H., Strelets, L., Brekke, O. H., Sandlie, I., and Michaelsen, T. E.  Comparisons of the ability of human IgG3 hinge mutants, IgM, IgE and IgA2 to form small immune complexes: a role for flexibility and geometry.  J. Immunol. 161: 4083-4090, 1998.

 

63.             Roux, K. H., Greenberg, A. S., Green, L., Strelets, L., Avila, D., McKinney, E. C. and Flajnik. M. F.  Structural analysis of the nurse shark (new) antigen receptor (NAR): molecular convergence of NAR and unusual mammalian immunoglobulins.  Proc. Natl. Acad. Sci.  95: 11804-11809, 1998.

 

64.             Acosta, M. R., Roux, K. H., Teuber, S. S. and Sathe, S. K.  Production and characterization of rabbit polyclonal antibodies to almond (Prunus amygdalus L.) major storage protein. J. Agric. Food Chem. 47, 4053-4059, 1999.

 

65.             Howard, J. T., Ward, J., Womer, J. and Roux, K. H.  Heteroduplex cleavage analysis using S1 nuclease. BioTechniques 27:18-19, 1999.

 

66.             Roux, K. H. Immunoglobulin structure and function as revealed by electron microscopy.  Int. Arch. Allergy Immunol. 120: 85-99, 1999. (An invited review)

 

67.             Schiller, D. S., Binley, J. M., Roux, K. H., Adamson, C. S., Jones, I. M., Krausslich, H-G. Hurley, A., Markowitz, M., and Moore, J. P.  Parameters influencing measurement of Gag antigen-specific T-proliferative response to HIV-1 infection. AIDS Res. Human Retroviruses 16: 259-271, 2000.

 

68.             Sathe, S. K., Teuber, S. S., Gradziel, T. M. and Roux, K. H.  Electrophoretic and immunological analyses of almond (Prunus dulcis L.) genotypes and hybrids. J. Agric. Food Chem. 49: 2043-2052. 2001

 

69.             Roux, K. H., Teuber, S. S., Robotham, J. M., and Sathe, S. K.  Detection and stability of the major almond allergen in foods. J. Agric. Food Chem. 49: 2131-2136, 2001. (

 

70.             Sze-Tao, K. W. C., Schrimpf, J. E., Teuber, S. S., Roux, K. H., and Sathe, S. K. Effects of processing and storage on walnut (Jugulans regia L.) tannins. J. Sci. Food Agric. 81:1215-1222, 2001.

 

71.             Zhu, P., Olson, W. C., and Roux, K. H. Structural flexibility and functional valency of CD4-IgG2 (PRO 542): potential for cross-linking Human Immunodeficiency Virus Type 1 envelope spikes.  J. Virol. 75: 6682-6686. 2001

 

72.             Laffer, S., Hogbom, E., Roux, K. H., Sperr, W. R., Valent, P., Bankl, H. C., Vangelista, L., Kricek, F., Kraft, D., Gronlund, H., and Valenta, R.  A molecular model of type I allergy: Identification and characterization of a nonanaphylactic anti-human IgE antibody fragment that blocks the IgE-Fc epsilon RI interaction and reacts with receptor-bound IgE. J. Allergy Clin. Immunol. 108:409-416, 2001.

 

73.             Robotham, J. M., Teuber, S. S., Sathe, S. K., and Roux, K. H.  Linear IgE epitope mapping of the English walnut (Juglans regia) major food allergen, Jug r 1. J. Allergy Clin. Immunol. 109: 143-149, 2002.

 

74.             Johansson, A., Erlandsson, Eriksson, D, Ullen, A, Holm, P, Sundstrom, B. E., Roux, K. H., and Stigbrand, T.  Idiotypic-anti-idiotypic complexes and their in vivo metabolism. Cancer 94:1306-1313, 2002.

 

75.             Wang, F., Robotham, J. M., Teuber, S. S., Tawde, P., Sathe, S. K., and Roux, K. H.  Ana o 1, a cashew (Anacardium occidentale) allergen of the vicilin seed storage protein family. J. Allergy Clin. Immunol. 110:160-166. 2002.

 

76.             Venkatachalam, M., Teuber, S. S., Roux, K. H., and Sathe, S. K.  Effects of roasting, blanching, autoclaving, and microwave heating on antigenicity of almond (Prunus dulcis L.) proteinsa. J Agric Food Chem. 50:3544-3548. 2002

 

77.             Roux K. H.  Single-step PCR optimization using touchdown and stepdown PCR programming.  Methods Mol Biol 2002;192:31-6. (co-listed and co-published as techniques book chapter)

 

78.             Schülke, N., Vesanen, M. S., Sanders, R. W., Zhu, P., Lu, M.,. Anselma, D. J.,. Villa, A. R.,. Parren, P. W. H. I., Binley, J. M., Roux, K. H., Maddon, P. J., Moore, J. P., Olson, W. C.  Oligomeric and conformational properties of a proteolytically mature, disulfide-stabilized human immunodeficiency virus type 1 gp140 envelope glycoprotein. J. Virol. 76:7760-7776. 2002.

 

79.             Sathe, S. K., Wolf, W. J., Roux, K. H., Teuber, S. S., Venkatachalam, M., and Sze-Tao, K. W. C.  Biochemical characterization of Amandin, the major storage protein in almond (Prunus dulcis L.).  J. Agric. Food Chem. 50:4333-4341. 2002.

 

80.             Teuber, S, S., Sathe, S. K., Peterson, W. R., and Roux, K. H.  Characterization of the soluble allergenic proteins of cashew nut (Anacardium occidentale L.) J. Agric. Food Chem. 50:6543-6549. 2002.

 

81.             Ferrant, J. L., Wilson, C., Benjamin, C., Hsu. Y-M, Karpusas, M., Roux, K. H., and Taylor, F. R.  Variation in the ordered structure of complexes between CD154 and anti-CD154 monoclonal antibodies. Molec. Immunol. 39:77-84. 2002.

 

82.             #Teuber, S. S. Comstock, S. S., Sathe, S. K., and Roux, K. H.  Tree nut allergy.  Curr Allergy Asthma Rep. 3:54-61. 2003. (An invited review)

 

83.             Wei, Y., Shridhar K. Sathe, S. K., Teuber, S. S., and. Roux, K. H.  A sensitive sandwich ELISA for the detection of trace amounts of cashew (Anacardium occidentale) nut in foods.  J. Agric. Food Chem. 51:3215-21. 2003.

 

84.             Calarese, D. A., Scanlan, C. N., Zwick, M. B., Deechongkit, S., Mimura, Y., Kunert, R., Zhu, P., Wormald, M. R., Stanfield, R. L., Roux, K. H., Kelly, J. W., Rudd, P. M., Dwek, R. A., Katinger, H., Burton, D. R., and Wilson, I. A.  Antibody domain exchange is an immunological solution to carbohydrate cluster recognition.  Science 300:2065-2071. 2003.)

 

85.             Wang, F., Robotham, J. M., Teuber, S. S., Sathe, S. K., and Roux, K. H.  Ana o 2, a major cashew nut (Anacardium occidentale L.) allergen of the legumin family.  Inter. Arch. Allergy Immunol. 132:  27-39, 2003.

 

86.             Roux, K. H., Teuber, S. S. and Sathe, S. K. Tree nut allergens. Int. Ach. Allergy Immunol.       131:234–244. 2003. (An invited review)

 

87.             Srivastava, I. K., Stamatatos, L, Kan, E., Lian, Y., Hilt, S., Martin, L., Vita, C., Zhu, P., Roux, K. H., Vojtech, L., Wininger, M., Ulmer, J. B., and Barnett, S. W.  Purification and characterization of a soluble trimeric envelope protein containing a partial deletion of the V2 loop derived from SF162, an R5-tropic HIV-1 isolate.  J. Virology 77:11244-11259. 2003.

 

88.             Zhu, P., Chertova, E., Julian Bess Jr., J., Lifson, J. D., Arthur, L. O., Liu, J., Taylor, K. A., and Roux, K. H.  Electron tomography analysis of envelope glycoprotein trimers on HIV and SIV virions.  Proc. Natl. Acad. Sci. USA, 100, 15812-15817, 2003.

 

89.             Su, M., Venkatachalam, M., Teuber, S. S., Roux, K. H., and Sathe, S. K., Impact of g-irradiation and thermal processing on the antigenicity of almond, cashew nut and walnut proteins. J. Sci. Food Agric. 84:  1119-11125, 2004.

 

90.             Løset, G. Å., Roux, K. H., Zhu, P., Michaelsen, T. E., and Sandlie, I.  Differential segmental flexibility and reach dictate the antigen binding mode of chimeric IgD and IgM:  implications for the function of the B cell receptor.  J. Immunol. 172:  2925-2934, 2004.

 

91.             Roux, K. H., Zhu, P. Seavy, M., Katinger, H., Kunert, R., Seamon, V. Electron microscopic and immunochemical analysis of the broadly neutralizing HIV-1-specific, anti-carbohydrate antibody, 2G12. Mol. Immunol. 41:  1001-1011, 2004.

 

92.             Wallowitz, M. L., Comstock, S. S., Roux, K. H., Sathe, S. K., and Teuber, S. K. Walnut Allergens. Food Allergy and Intolerance, 5: 187-195, 2004.

 

93.                   Robotham, J. M., Wang, F., Seamon, V, Teuber, S. S., Sathe, S. K., Beyer, K, Sampson, H. and Roux, K. H.  Ana o 3, an important cashew nut (Anacardium occidentale L.) allergen of the 2S albumin family. J. Allergy Clin. Immunol. 15:  1284-1290, 2005.

 

94.                   Pancera, M., Lebowitz, J., Schön, A., Zhu, P., Freire, E., Kwong, P. D., Roux, K. H., Sodroski, J. and Wyatt, R.  Soluble mimetics of human immunodeficiency virus type 1 viral spikes produced by replacement of the native trimerization domain with a heterologous trimerization motif: characterization and ligand binding analysis. J. Virol., 79:  9954-9969, 2005.

 

95.                   Sathe, S. K., Teuber, S. S., Roux, K. H.  Effects of food processing on the stability of food allergens.  Biotech. Adv. 232:  423-429, 2005.

 

96.                   Sathe, S. K., Kshirsagar, H. H., and Roux, K. H.  Advances in seed protein research:  a perspective on seed allergens. J. Food Science, 70:  93-120, 2005.

 

97.                   Lee, S-H, Benmoussa, M., Sathe, S. K., Roux, K. H., Teuber, S. S., and Hamaker, B. R. A 50 kDa maize γ-zein has marked cross-reactivity with the almond major allergen. J. Agric. Food Chem., 53:  7965-7970, 2005

 

98.                   Moore,.P. L., Crooks, E. T. Porter, L, Zhu, P., Cayanan, C. S., Grise, H, Corcoran, P., Zwick, M. B., Franti, M., Morris, L., Roux, K. H.,. Burton, D. R., and Binley, J. M.  Nature of nonfunctional envelope proteins on the surface of human immunodeficiency virus type-1. J. Virol. 80:  2515-2528, 2006.

 

99.                   Venkatachalam, M., Teuber, S. S., Roux, K. H., and Sathe, S. K.  Antigenic stability of pecan [Carya illinoinensis (Wangenh.) K. Kock] proteins: effects of thermal treatments and in vitro digestion. J. Ag. Food Chem. 54:  1449-1458, 2006.

 

100.               Zhu, P. Liu,J., Bess Jr., J., Chertova, E., Lifson, J. D., Grise, H., Ofek, G., Taylor, K. A., and Roux, K. H. Distribution and three-dimensional structure of AIDS virus envelope spikes. Nature, 441:  847-852, 2006.  (Featured in News & Views section: Burton, D. R., Images from the surface of HIV. Nature 441: 847-852, 2006.

 

101.               Tawde, P., Venkatesh, Y. P., Wang, F., Teuber, S. S., Sathe, S, K., and Roux, K. H.  Cloning and characterization of profilin (Pru du 4), a cross-reactive almond (Prunus dulcis) allergen. J. Allergy Clin. Immunol. 118:915-922. 2006.

 

102.               Zhu, P., Taylor, K., and Roux, K.  Structure of AIDS virus surface revealed: Cryoelectron tomography shows 3D structure of surface spikes. BIOforum Europe 7/8 32-33. 2006.