Supplemental 3D Deconvolution Image Data of histone H2B-mCherry.

 

These movies are derived from five different multi-wavelength image datasets.

 

(2012) Howe ES, Clemente TE, and Bass HW

"Maize histone H2B-mCherry, a new fluorescent chromatin marker for
somatic and meiotic chromosome research."

DNA and Cell Biology. 31(6):925-938 ( DOI: 10.1089/dna.2011.1514).

 

 

Movie File Name (.mov files on gdocs)

Cell type

Figure

Description

H2BmC01_Xylem_Dapi

Somatic,
cell with xylem

5C

grey-scale sections,
DAPI  (DNA)

H2BmC01_Xylem_Rhod

"

5D

grey-scale sections,
RHOD  (H2B-mCherry)

H2BmC01_Xylem_DapiRhod

"

5E

color sections,
DAPI/RHOD = Red/Green

H2BmC01_Xylem_DapiRhod_360

"

5E

color, spinning projections, DAPI/RHOD = Red/Green

 

 

 

 

H2BmC01_Pachytene_Dapi

Male meiotic Prophase Pachytene

-

grey-scale sections,
DAPI  (DNA)

H2BmC01_Pachytene_Dapi_360

"

-

grey-scale , spinning projections, DAPI (DNA)

H2BmC01_Pachytene_Rhod

"

-

grey-scale sections,
RHOD  (H2B-mCherry)

H2BmC01_Pachytene_Rhod_360

"

-

grey-scale , spinning projections, RHOD (H2B-mCherry)

H2BmC01_Pachytene_DapiRhod

"

-

color sections,
DAPI/RHOD = Red/Green

H2BmC01_Pachytene_DapiRhod_360

"

-

color, spinning projections, DAPI/RHOD = Red/Green

 

 

 

 

H2BmC01_TeloI_Dapi_360

Male meiosis Telophase I

7G

grey-scale , spinning projections, DAPI (DNA)

H2BmC01_TeloI_Rhod_360

"

7H

grey-scale , spinning projections, RHOD (H2B-mCherry)

 

 

 

 

H2BmC01_LateUniNuc_Dapi

Gametophyte, late uninucleate

8G

grey-scale sections,
DAPI  (DNA)

H2BmC01_LateUniNuc_Dapi_360

"

8G

grey-scale , spinning projections, DAPI (DNA)

H2BmC01_LateUniNuc_Rhod

"

8H

grey-scale sections,
RHOD  (H2B-mCherry)

H2BmC01_LateUniNuc_Rhod_360

"

8H

grey-scale , spinning projections, RHOD (H2B-mCherry)

H2BmC01_LateUniNuc_DapiRhod

"

8I

color sections,
DAPI/RHOD = Red/Green

H2BmC01_LateUniNuc_DapiRhod_360

"

8I

color, spinning projections,
DAPI/RHOD = Red/Green

 

 

 

 

H2BmC01_PollenUniNuc_Dapi

Early pollen uninucleate

8J

grey-scale sections,
DAPI  (DNA)

H2BmC01_PollenUniNuc_Rhod

"

8K

grey-scale sections,
RHOD  (H2B-mCherry)

H2BmC01_PollenUniNuc_DapiRhod

"

8L

color sections,
DAPI/RHOD = Red/Green

H2BmC01_PollenUniNuc_DapiRhod_360

"

8L

color, spinning projections, DAPI/RHOD = Red/Green

9/2012 (contact: bass@bio.fsu.edu)