ABSTRACT

Ring, B.C., Bass, H.W., and Garza, D. (2000)
  Construction and Transposition of a 100-kilobase "Extended" P element
  in Drosophila. Genome Research10(10):1605-1616.
(Abstract), (PubMed Abs), (Genome Research online).

Abstract.
  We have used P element deletion derivatives at defined locations in the 
Drosophila genome to construct a 100-kilobase "extended" P element, 
more than twice the size of any previously available.  We demonstrate that 
this prototypical extended P element is capable of transposition to new sites 
in the genome.  The structural and functional integrity of a transposed 
extended P element was confirmed using molecular, genetic, and cytogenetic 
criteria.  This is the first method shown to be capable of producing large, 
unlinked transpositional duplications in Drosophila.  The ability to produce 
functional transposable elements from half-elements is novel and has many 
potential applications for the functional analysis of complex genomes.

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